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Representative histopathological images of the neurocytoma. A: Hematoxylin-eosin. B: Insulinoma-associated protein 1 immunohistochemical reaction. Brown cells are positive for INSM1, which is an accepted marker for neurocytoma. C: Representative neurocytoma section showing the absence of cells immunostained for oxytocin. D: Representative neurocytoma section showing the absence of cells immunostained for tyrosine hydroxylase. We used the following antibodies on formalin-fixed paraffin-embedded 8-µm sections: INSM1 (rabbit anti-INSM1 [SP493] monoclonal antibody, concentration 5 µg/ml, Ventana-Roche), OXT (rabbit anti-oxytocin polyclonal antibody, dilution 1:5000, Merck AB911), and TH <t>(chicken</t> <t>anti–tyrosine</t> hydroxylase polyclonal antibody, dilution <t>1:1000,</t> Merck <t>AB9702).</t> After immunohistochemical reaction, the sections were lightly counterstained with hematoxylin. Scale bars = 10 µm.
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Representative histopathological images of the neurocytoma. A: Hematoxylin-eosin. B: Insulinoma-associated protein 1 immunohistochemical reaction. Brown cells are positive for INSM1, which is an accepted marker for neurocytoma. C: Representative neurocytoma section showing the absence of cells immunostained for oxytocin. D: Representative neurocytoma section showing the absence of cells immunostained for tyrosine hydroxylase. We used the following antibodies on formalin-fixed paraffin-embedded 8-µm sections: INSM1 (rabbit anti-INSM1 [SP493] monoclonal antibody, concentration 5 µg/ml, Ventana-Roche), OXT (rabbit anti-oxytocin polyclonal antibody, dilution 1:5000, Merck AB911), and TH <t>(chicken</t> <t>anti–tyrosine</t> hydroxylase polyclonal antibody, dilution <t>1:1000,</t> Merck <t>AB9702).</t> After immunohistochemical reaction, the sections were lightly counterstained with hematoxylin. Scale bars = 10 µm.
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Representative histopathological images of the neurocytoma. A: Hematoxylin-eosin. B: Insulinoma-associated protein 1 immunohistochemical reaction. Brown cells are positive for INSM1, which is an accepted marker for neurocytoma. C: Representative neurocytoma section showing the absence of cells immunostained for oxytocin. D: Representative neurocytoma section showing the absence of cells immunostained for tyrosine hydroxylase. We used the following antibodies on formalin-fixed paraffin-embedded 8-µm sections: INSM1 (rabbit anti-INSM1 [SP493] monoclonal antibody, concentration 5 µg/ml, Ventana-Roche), OXT (rabbit anti-oxytocin polyclonal antibody, dilution 1:5000, Merck AB911), and TH <t>(chicken</t> <t>anti–tyrosine</t> hydroxylase polyclonal antibody, dilution <t>1:1000,</t> Merck <t>AB9702).</t> After immunohistochemical reaction, the sections were lightly counterstained with hematoxylin. Scale bars = 10 µm.
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Representative histopathological images of the neurocytoma. A: Hematoxylin-eosin. B: Insulinoma-associated protein 1 immunohistochemical reaction. Brown cells are positive for INSM1, which is an accepted marker for neurocytoma. C: Representative neurocytoma section showing the absence of cells immunostained for oxytocin. D: Representative neurocytoma section showing the absence of cells immunostained for tyrosine hydroxylase. We used the following antibodies on formalin-fixed paraffin-embedded 8-µm sections: INSM1 (rabbit anti-INSM1 [SP493] monoclonal antibody, concentration 5 µg/ml, Ventana-Roche), OXT (rabbit anti-oxytocin polyclonal antibody, dilution 1:5000, Merck AB911), and TH (chicken anti–tyrosine hydroxylase polyclonal antibody, dilution 1:1000, Merck AB9702). After immunohistochemical reaction, the sections were lightly counterstained with hematoxylin. Scale bars = 10 µm.

Journal: Journal of Neurosurgery: Case Lessons

Article Title: Intraventricular neurocytoma presenting as Kleine-Levin syndrome: resolution after resection and review of neurosurgical conditions associated with hypersexuality. Illustrative case

doi: 10.3171/CASE25800

Figure Lengend Snippet: Representative histopathological images of the neurocytoma. A: Hematoxylin-eosin. B: Insulinoma-associated protein 1 immunohistochemical reaction. Brown cells are positive for INSM1, which is an accepted marker for neurocytoma. C: Representative neurocytoma section showing the absence of cells immunostained for oxytocin. D: Representative neurocytoma section showing the absence of cells immunostained for tyrosine hydroxylase. We used the following antibodies on formalin-fixed paraffin-embedded 8-µm sections: INSM1 (rabbit anti-INSM1 [SP493] monoclonal antibody, concentration 5 µg/ml, Ventana-Roche), OXT (rabbit anti-oxytocin polyclonal antibody, dilution 1:5000, Merck AB911), and TH (chicken anti–tyrosine hydroxylase polyclonal antibody, dilution 1:1000, Merck AB9702). After immunohistochemical reaction, the sections were lightly counterstained with hematoxylin. Scale bars = 10 µm.

Article Snippet: We used the following antibodies on formalin-fixed paraffin-embedded 8-μm sections: INSM1 (rabbit anti-INSM1 [SP493] monoclonal antibody, concentration 5 μg/ml, Ventana-Roche), OXT (rabbit anti-oxytocin polyclonal antibody, dilution 1:5000, Merck AB911), and TH (chicken anti–tyrosine hydroxylase polyclonal antibody, dilution 1:1000, Merck AB9702).

Techniques: Immunohistochemical staining, Marker, Formalin-fixed Paraffin-Embedded, Concentration Assay